Instruments

Schedule Use

Trained users may sign up to use any of the analyzers 24 hours a day/7 days a week by clicking here to login to the portal. The MoFlo Astrios cell sorter and the FACSAria Fusion are operated by laboratory personnel only.  Contact Julie Nelson for an appointment on the MoFlo Astrios and Jamie Barber for an appointment on the FACSAria Fusion. All instruments are located in room S166 Paul D. Coverdell unless otherwise noted.

We recommend Fluorofinder for building panels to use for these instruments

Beckman Coulter CyAn

The CyAn has three laser excitation wavelengths, 405, 488 and 610, that are capable of analyzing 11 parameters simultaneously at speeds up to 20,000 events/second. These cytometers are useful for those searching for rare cells and those with applications requiring more than 4-5 colors to define a cell population. The CyAn is designed to be user friendly and most investigators are comfortable with it after one or more training sessions.  We have 2 CyAns located in the Paul D. Coverdell location; on iLab they are listed as the CyAn and the HyperCyAn.

CyAn equipment

Beckman Coulter CytoFLEX

The CytoFLEX (Beckman Coulter) has four laser excitation wavelengths, 405, 488, 561 and 610, that are capable of analyzing 13 parameters simultaneously.  This unique cytometer uses WDMs (What are WDMs?) and APDs (What are APDs? ) to increase sensitivity of detection.  The CytoFLEX can sample from a 96 well plate and give you a concentration of cells without using beads.  This cytometer is useful for measuring fluorescent proteins that require yellow, 561 nm, excitation such as mCherry, mPlum and dsRed. The CytoFLEX is designed to be user friendly and most investigators are comfortable with it after one or more training sessions.  We have 2 CytoFLEX instruments available: 1 in the Paul D. Coverdell location and 1 in the CMM location, listed as CytoFLEX and CMM CytoFLEX respectively in iLab.

Cytoflex equipment

Beckman Coulter MoFlo Astrios EQ

The MoFlo Astrios EQ (Beckman Coulter) cell sorter purifies or enriches populations by sorting cells or particles based on 20 different parameters using either or all of the following excitation wavelengths – 355, 405, 488, 561, or 640. The MoFlo Astrios can sort at speeds up to 60,000 events/second and can sort single cells into any multi-well platform. The MoFlo Astrios is in a BSL2 Biosafety Cabinet for sorting infectious cells. Researchers interested in using the MoFlo Astrios should contact Julie Nelson to schedule a consultation.

MoFlo Astrios in Hood

The Bio-Rad S3

The (Bio-Rad) S3 cell sorter purifies or enriches populations by sorting cells or particles based on 6 different parameters (4 colors/2 scatter) using either or both of the following excitation wavelengths – 488 or 561 The S3 sorts in 2 directions in to either a 5 ml snap cap tube or microcentrifuge tube. This instrument is designed to be user-friendly after training.  Researchers interested in using the S3 should contact Julie Nelson to schedule training.

lab equipment

Becton Dickinson FACSMelody

The FACSMelody (Becton Dickinson) cell sorter purifies or enriches populations by sorting cells or particles based on 10 different parameters (8 colors/2 scatter) using either or all of the following excitation wavelengths – 488, 561, 634. The FACSMelody can sort up to 4 populations in to 5 ml snap cap tubes, microcentrifuge tubes, slides or 96 well plates. This instrument is designed to be user-friendly after training. It is located in room 2231C of the CMM and researchers interested in using it should contact Michael Kulik to schedule training.

FACS Melody equipment

Becton Dickinson FACSAria Fusion

The FACSAria Fusion (Becton Dickinson) contains 4 lasers: red (640nm), yellow (561nm), blue (488nm) and violet (405nm). In its present configuration, the sorter can evaluate thirteen parameters (forward and side scatter plus eleven fluorescent channels). It has the ability to sort single cells into 6, 24, 48, 96- or 384-well plates. This instrument also has an aerosol management system to eliminate the emission of aerosols generated during operation. Further, the FACSAria Fusion is designed for the sorting of infected samples and will be operated by individuals wearing respirators to further decrease the potential for contact with infectious aerosols. In addition, the entire instrument is contained in a laminar flow biosafety cabinet that is certified annually. This instrument is recommended for users who need to sort infectious, BSL2 particles. The FACSAria Fusion is located in the CVI, room 1557 and researchers interested in using it should contact Jamie Barber to schedule a consultation.

FACSAria Fusion equipment

Bio-Rad Bio-Plex

The Bio-Plex multiplex system enables the detection and quantification of multiple analytes (proteins and peptides, or nucleic acids) in a single sample volume. Utilizing xMAP technology licensed from Luminex, the Bio-Plex Multiplex system can multiplex up to 500 different assays simultaneously.

Bio-Plex equipment

Millipore Sigma ImageStream Mark II

The ImageStream (Millipore Sigma) is an imaging cytometer.  This instrument combines the speed, sensitivity, and phenotyping abilities of flow cytometry with the detailed imagery and functional insights of microscopy. This unique combination enables a broad range of applications that would be impossible using either technique alone.

This instrument produces multiple high-resolution images of every cell directly in flow, including brightfield and darkfield (SSC), and up to 10 fluorescent markers (with 4 laser excitation – 405nm, 488nm, 561nm and 642nm) with sensitivity exceeding conventional flow cytometers.  The ImageStream is designed to be user-friendly after 1-2 training sessions.  Interested users should contact Julie Nelson.

Image Stream equipment

Nexcelom Bioscience Cellometer X2

The Cellometer uses bright field and fluorescence imaging combined with sophisticated image analysis software to yield accurate, hassle free calculations of everything from the basic cell count, size, and viability measurements. The instrument can count most common sample types, from cell lines to primary cells to yeast, algae, and blood platelets, and it will allow users to view, print, and save every image captured.

Cellometer equipment
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