The Quanteon (Agilent) analyzer has four laser excitation wavelengths, 405, 488, 561 and 640, that are capable of analyzing 25 parameters simultaneously. This unique cytometer uses siPMs (What is a siPM?) providing the latest in sensitivity of detection. The Quanteon can sample from any platform type plate (96 well, 384 well, 96 deep well, 12 well, etc.) or tube (1.5 ml Eppendorf or 5 ml round bottom) in an automated fashion. With its superior fluidics that provide pulse-free sample delivery the user can obtain absolute counts, without the use of expensive beads. This cytometer is useful for measuring fluorescent proteins that require yellow, 561 nm, excitation such as mCherry, mPlum and dsRed as well as high dimensional immunophenotyping. The Quanteon is designed to be user friendly and most investigators are comfortable with it after one or more training sessions. We have 2 Quanteon instruments available on campus: 1 in the Paul D. Coverdell location (contact Julie Nelson for training) and 1 in the CVM (contact Jamie Barber for training).